Strain Data Sheet

RBRC04416

Strain Information

Image
BRC No.RBRC04416
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Fam60a<tm1Hmd>/226
Former Common nameSap35 KO#226(flox/flox), B6;129-Sap35<tm1Hmd>/226
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yasuo Sakai and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2001.
Strain description
Colony maintenance
References
Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters.
Ryo Nabeshima, Osamu Nishimura, Takako Maeda, Natsumi Shimizu, Takahiro Ide, Kenta Yashiro, Yasuo Sakai, Chikara Meno, Mitsutaka Kadota, Hidetaka Shiratori, Shigehiro Kuraku, Hiroshi Hamada
Elife, 7:e36435 (2018). 30070635

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Sinhcaf
MGI:1929091		SIN3-HDAC complex associated factor6Sinhcaf<tm1Hmd> targeted mutation 1, Hiroshi Hamada
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Sinhcaf genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eLife pii: e36435 (2018).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data