Strain Data Sheet

RBRC04418

Strain Information

Image
BRC No.RBRC04418
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6C3-Tg(Barh1-lacZ)2Tets
Former Common nameB6.B6C3-Tg(Barh1-lacZ)2Tetsu, Barh1-lacZ transgenic mouse 2, Mbh1-lacZ Tg2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tetsuichiro Saito, Graduate School of Medicine, Chiba University in 2001.
Strain descriptionlacZ is expressed in developing spinal commissural neurons, cerebellar granule cells and the retina, where Barh1 (Mbh1) is expressed.
Colony maintenance
References
Mammalian BarH1 confers commissural neuron identity on dorsal cells in the spinal cord.
Saba R, Nakatsuji N, Saito T
J. Neurosci., 23, 1987-1991 (2003). 12657654

Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1.
Saba R, Johnson J E, Saito T
Development, 132, 2147-2155 (2005). 15788459

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
lacZ beta-galactosidase (E. coli)UN mouse Barh1 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ gene, Mouse Barh1 genomic DNA containing promoter and enhancer, SV40 PolyA signal
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 132, 217-2155 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE is requested to submit the abstract of the research project to the DEPOSITOR and shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTetsuichiro Saito (Chiba University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data