Strain Data Sheet

RBRC04420

Strain Information

Image
BRC No.RBRC04420
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(SJL)-Tcirg1<tm1.1Ywd>
Former Common namea3-GFP, Tcirg1<floxGFP>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008. This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon.
Strain descriptionThis allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele.
Colony maintenance
References
Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification.
Sun-Wada G H, Tabata H, Kawamura N, Aoyama M, Wada Y
J. Cell Sci., 122, 2504-2513 (2009). 19549681

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site19Frt
GFP Green Fluorescent Protein (Aequorea victoria)19
Tcirg1
MGI:1350931		T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.1Ywd>
MGI:3852639		targeted mutation 1.1, Yoh Wada
  • autosomal recessive osteopetrosis 1(MedGEN)
    • loxP phage P1 loxP19loxP
    loxP phage P1 loxP19loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal fertility/fecundity(MP:0002161)

  • abnormal macrophage physiology(MP:0002451)

  • abnormal osteoclast physiology(MP:0001541)

  • cellular phenotype(MP:0005384)
    • more 2 phenotypes
  • decreased bone resorption(MP:0004993)

  • hydrocephaly(MP:0001891)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage phage P1 loxP sites, Yeast flipase recombination target site, Jellyfish GFP cDNA, Mouse Tcirg1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorYoh Wada (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data