Strain Data Sheet

RBRC04421

Strain Information

Image
BRC No.RBRC04421
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(FVB)-Tcirg1<tm1.2Ywd>
Former Common namea3 mutant, Tcirg1<delta>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008.
Strain descriptionThe gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. Homozygous mutant mice die within 3-4 weeks after birth.
Colony maintenance
References
Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification.
Sun-Wada G H, Tabata H, Kawamura N, Aoyama M, Wada Y
J. Cell Sci., 122, 2504-2513 (2009). 19549681

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tcirg1
MGI:1350931		T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.2Ywd>
MGI:3852642		targeted mutation 1.2, Yoh Wada
  • autosomal recessive osteopetrosis 1(MedGEN)
    • loxP phage P1 loxP19loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal macrophage physiology(MP:0002451)

  • absent teeth(MP:0030610)

  • decreased body size(MP:0001265)

  • obsolete absent teeth(MP:0000124)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage phage P1 loxP site, Mouse Tcirg1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorYoh Wada (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Matsumoto N, Sekiya M, Sun-Wada GH, Wada Y, Nakanishi-Matsui M.
    The lysosomal V-ATPase a3 subunit is involved in localization of Mon1-Ccz1, the GEF for Rab7, to secretory lysosomes in osteoclasts.
    Sci Rep 12(1) 8455(2022) 35589873
    Matsumoto N, Nakanishi-Matsui M.
    Proton pumping V-ATPase inhibitor bafilomycin A1 affects Rab7 lysosomal localization and abolishes anterograde trafficking of osteoclast secretory lysosomes.
    Biochem Biophys Res Commun 510(3) 421-426(2019) 30717974