Strain Data Sheet

RBRC04425

Strain Information

Image
BRC No.RBRC04425
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-Nup85*DN)6-1Sush
Former Common nameFNT-Two-Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kouji Matsushima, Graduate School of Medicine, University of Tokyo.
Strain description
Colony maintenance
References
Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis.
Terashima Y, Onai N, Murai M, Enomoto M, Poonpiriya V, Hamada T, Motomura K, Suwa M, Ezaki T, Haga T, Kanegasaki S, Matsushima K
Nat. Immunol., 6, 827-835 (2005). 15995708

FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.
Toda E, Terashima Y, Sato T, Hirose K, Kanegasaki S, Matsushima K
J. Immunol., 183, 6387-6394 (2009). 19841162

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nup85
MGI:3046173		nucleoporin 85UNNup85 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • nephrotic syndrome, type 17(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Mouse Nup85 cDNA (dominant negative type)
    Research application
    Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKouji Matsushima (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data