Strain Data Sheet

RBRC04434

Strain Information

Image
BRC No.RBRC04434
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Msi1<tm1Okn>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinsuke Shibata and Hideyuki Okano, Keio University School of Medicine.
Strain descriptionC57BL/6J background Msi1 deficient mice (RBRC04434) frequently died of obstructive hydrocephalus within a couple of month with aberrant cell proliferation around cerebral aqueduct. Both male and female are infertile because of the early death of hydrocephalus. On the other hand, CD-1 (ICR) background Msi1 deficient mice (RBRC04435) survive to adult without gross anatomical phenotype. Since both male and female are fertile, it is possible to mate between Msi1 null mice.
Colony maintenance
References
RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation.
Sakakibara S, Nakamura Y, Yoshida T, Shibata S, Koike M, Takano H, Ueda S, Uchiyama Y, Noda T, Okano H
Proc. Natl. Acad. Sci. USA, 99, 15194-15199 (2002). 12407178

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Msi1
MGI:107376		musashi RNA-binding protein 15Msi1 targeted mutation 1, Hideyuki Okano
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cerebral cortex morphology(MP:0000788)

  • abnormal corpus callosum morphology(MP:0000780)

  • abnormal ependyma morphology(MP:0002653)

  • abnormal neurocranium morphology(MP:0000074)

  • ataxia(MP:0001393)
    • more 6 phenotypes
  • dehydration(MP:0001429)

  • dilated lateral ventricle(MP:0000825)

  • edema(MP:0001785)

  • hydrocephaly(MP:0001891)

  • intracerebral hemorrhage(MP:0001916)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Musashi1 genomic DNA, E. coli Tn5neo gene, mouse PGK promoter, SV40 polyA
    Research applicationCell Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 15194-15199 (2002).For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorHideyuki Okano (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data