Strain Data Sheet

RBRC04436

Strain Information

Image
BRC No.RBRC04436
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Msi2<tm1Okn>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinsuke Shibata and Hideyuki Okano, Keio University School of Medicine.
Strain descriptionMsi2 deficient mice (RBRC04436 and RBRC00437) survive to adult and show poor weight gain, low spontaneous movement and sensory dysfunction, with abnormal PNS development. The cause of the phenotype is thought to be poor neural fiber innervations of specific sensory neuron from DRG to spinal cord. These phenotypes are observed both background (C57BL/6J and CD-1) and detailed analyses were performed in CD-1 background. Msi2 deficient male mice show azoospermia and are infertile. Because the movement of sperm taken from epidermis of Msi2 null male was bad, in vitro fertilization was not successful. Msi2 deficient female mice are fertile; however, probability of pregnancy is poor presumably due to the low spontaneous activity.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Msi2
MGI:1923876		musashi RNA-binding protein 211Msi2<tm1Okn> targeted mutation 1, Hideyuki Okano
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11
poly A polyA11poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal motor capabilities/coordination/movement(MP:0002066)

  • abnormal sensory neuron morphology(MP:0000965)

  • decreased body weight(MP:0001262)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Musashi2 genomic DNA, E. coli Tn5neo gene, mouse PGK promoter, SV40 PolyA
    Research applicationCell Biology Research
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorHideyuki Okano (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data