Strain Data Sheet

RBRC04488

Strain Information

Image
BRC No.RBRC04488
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129P2(B6)-Sirpa<tm1Nog>
Former Common nameSIRP alpha mutant mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Kenjiro Inagaki at Kobe University in 2000. A neomycin selection cassette replaced exons 7 and 8 of the M83 gene. The mutant mice were backcrossed C57BL/6N for 5 times, and then backcrossed to BALB/c over 15 times.
Strain descriptionSHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene is a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.
Colony maintenance
References
SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility.
Inagaki K, Yamao T, Noguchi T, Matozaki T, Fukunaga K, Takada T, Hosooka T, Akira S, Kasuga M
EMBO J., 19, 6721-6731 (2000). 11118207

Negative regulation of platelet clearance and of the macrophage phagocytic response by the transmembrane glycoprotein SHPS-1.
Takuji Yamao, Tetsuya Noguchi, Osamu Takeuchi, Uichi Nishiyama, Haruhiko Morita, Tetsuya Hagiwara, Hironori Akahori, Takashi Kato, Kenjiro Inagaki, Hideki Okazawa, Yoshitake Hayashi, Takashi Matozaki, Kiyoshi Takeda, Shizuo Akira, Masato Kasuga
J. Biol. Chem., 277 (42) 39833-9 (2002). 12167615

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sirpa
MGI:108563		signal-regulatory protein alpha2Sirpa<tm1Nog>
MGI:2388979		targeted mutation 1, Tetsuya Noguchi
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Langerhans cell physiology(MP:0005362)

  • abnormal macrophage physiology(MP:0002451)

  • behavioral despair(MP:0002573)

  • decreased T cell number(MP:0005018)

  • decreased body weight(MP:0001262)
    • more 3 phenotypes
  • hepatic steatosis(MP:0002628)

  • increased megakaryocyte cell number(MP:0008254)

  • thrombocytopenia(MP:0003179)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, PGK PolyA, mouse Sirpa genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT f BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTakashi Matozaki (Gunma University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data