Strain Data Sheet

RBRC04489

Strain Information

Image
BRC No.RBRC04489
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Hoil1<tm1Kiwa>
Former Common nameHOIL-1L KO, B6.B6CB-Hoil1<tm1Kiwa>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuhiro Iwai, Graduate School of Medicine, Osaka City University in 2004. TT2 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain descriptionRbck1 (HOIL-1L) knockout mice. Homozygous mutant mice show attenuated TNF-alpha mediated NF-kappaB activation.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
References
Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation.
Tokunaga F, Sakata S, Saeki Y, Satomi Y, Kirisako T, Kamei K, Nakagawa T, Kato M, Murata S, Yamaoka S, Yamamoto M, Akira S, Takao T, Tanaka K, Iwai K
Nat. Cell. Biol., 11, 123-132 (2009). 19136968

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rbck1
MGI:1344372		RanBP-type and C3HC4-type zinc finger containing 12Rbck1<tm1Kiwa>
MGI:5000222		Targeted mutation 1, Kazuhiro Iwai
neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased circulating tumor necrosis factor level(MP:0008554)

  • increased hepatocyte apoptosis(MP:0003887)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli Neomycin resistance gene, mouse Rbck1 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Cell. Biol., 11, 123-132 (2009). The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKazuhiro Iwai (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data