Strain Data Sheet

RBRC04503

Strain Information

Image
BRC No.RBRC04503
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Smad7<tm1Tchi>
Former Common nameSmad7 flox
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Masayoshi Tojo and Tomoki Chiba, Graduate School of Life and Environmental Sciences in 2007.
Strain descriptionHomozygous mutant mice are embryonic lethal.
Colony maintenance
References
Smad7-deficient mice show growth retardation with reduced viability.
Tojo M, Takebe A, Takahashi S, Tanaka K, Imamura T, Miyazono K, Chiba T
J Biochem., 151 (6): 621-631 (2012). 22383537

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site18Frt
Frt yeast FRT (flippase recombination target) site18Frt
Smad7
MGI:1100518		SMAD family member 718Smad7<tm1Tchi> targeted mutation 1, Tomoki Chiba
  • colorectal cancer, susceptibility to, 3(MedGEN)
    • loxP phage P1 loxP18loxP
    loxP phage P1 loxP18loxP
    neo neomycin resistance gene (E. coli)18 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, phage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse Smad7 genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for publication of research papers. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTomoki Chiba (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data