Strain Data Sheet

RBRC04549

Strain Information

Image
BRC No.RBRC04549
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Nmu<tm1Mko>
Former Common nameNMU-KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain descriptionNmu (Neuromedin U) gene knock-out mice. Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms gene knockout mice (RBRC04550), Nmu and Nms double knockout mice (RBRC04737).
Colony maintenanceHeterozygote × wild-type [or Crossing to C57BL/6JJcl]
References
Neuromedin U has a novel anorexigenic effect independent of the leptin signaling pathway.
Hanada R, Teranishi H, Pearson J T, Kurokawa M, Hosoda H, Fukushima N, Fukue Y, Serino R, Fujihara H, Ueta Y, Ikawa M, Okabe M, Murakami N, Shirai M, Yoshimatsu H, Kangawa K, Kojima M
Nat. Med., 10, 1067-1073 (2004). 15448684

The neuropeptide neuromedin U promotes inflammation by direct activation of mast cells.
Moriyama M, Sato T, Inoue H, Fukuyama S, Teranishi H, Kangawa K, Kano T, Yoshimura A, Kojima M
J. Exp. Med., 202, 217-224 (2005). 16009716

Health Report

Examination Date / Room / Rack2024/06/10Room:3-3Rack:BSentinel mouse program
2024/03/11Room:3-3Rack:BSentinel mouse program
2023/12/11Room:3-3Rack:BSentinel mouse program
2023/09/11Room:3-3Rack:BSentinel mouse program
2023/06/13Room:3-3Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nmu
MGI:1860476		neuromedin U5Nmu<tm1Mko>
MGI:3056512		targeted mutation 1, Masayasu Kojima
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circadian behavior(MP:0020467)

  • abnormal glucose homeostasis(MP:0002078)

  • abnormal lipid homeostasis(MP:0002118)

  • decreased circulating corticosterone level(MP:0002665)

  • decreased locomotor activity(MP:0001402)
    • more 12 phenotypes
  • decreased oxygen consumption(MP:0005290)

  • hepatic steatosis(MP:0002628)

  • hyperglycemia(MP:0001559)

  • impaired adaptive thermogenesis(MP:0011049)

  • increased circulating cholesterol level(MP:0005178)

  • increased circulating free fatty acids level(MP:0001554)

  • increased circulating insulin level(MP:0002079)

  • increased circulating leptin level(MP:0005669)

  • increased fat cell size(MP:0006094)

  • increased triglyceride level(MP:0005317)

  • obese(MP:0001261)

  • polyphagia(MP:0001433)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nmu genomic DNA
    Research applicationMetabolism Research
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorMsayasu Kojima (Kurume University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Wallrapp A, Riesenfeld SJ, Burkett PR, Abdulnour RE, Nyman J, Dionne D, Hofree M, Cuoco MS, Rodman C, Farouq D, Haas BJ, Tickle TL, Trombetta JJ, Baral P, Klose CSN, Mahlakõiv T, Artis D, Rozenblatt-Rosen O, Chiu IM, Levy BD, Kowalczyk MS, Regev A, Kuchroo VK.
    The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation.
    Nature 549(7672) 351-356(2017) 28902842