Strain Data Sheet
RBRC04553
Strain Information | |
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| Image | |
| BRC No. | RBRC04553 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Rrp8<tm1Jyan>/2Rbrc |
| Former Common name | NML-KO, B6.Cg-Nml<tm1>/2 |
| H-2 Haplotype | |
| ES Cell line | iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Akiko Murayama and Jun Yanagisawa, Life Science Center of Tsukuba Advanced Research Alliance, The University of Tsukuba in 2008. iTL ES cells derived from (C57BL/6 x 129)F1 were used. The mutant mice were crossed to C57BL/6 over 6 times. |
| Strain description | Nml (Nucleomethylin) gene knockout mice. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Epigenetic control of rDNA loci in response to intracellular energy status. Murayama A, Ohmori K, Fujimura A, Minami H, Yasuzawa-Tanaka K, Kuroda T, Oie S, Daitoku H, Okuwaki M, Nagata K, Fukamizu A, Kimura K, Shimizu T, Yanagisawa J Cell, 133, 627-639, (2008). 18485871The nucleolar protein NML regulates hepatic ATP levels during liver regeneration after partial hepatectomy. Mikogai A, Yanagisawa J, Yasuzawa-Tanaka K, Murayama A Biochem. Biophys. Res. Commun., 390, 591-596 (2009). 19819226Hepatic rRNA transcription regulates high-fat-diet-induced obesity. Oie S, Matsuzaki K, Yokoyama W, Tokunaga S, Waku T, Han S I, Iwasaki N, Mikogai A, Yasuzawa-Tanaka K, Kishimoto H, Hiyoshi H, Nakajima Y, Araki T, Kimura K, Yanagisawa J, Murayama A Cell Rep., 7(3)807-820 (2014). 24746822 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| Rrp8 MGI:1914251 | ribosomal RNA processing 8 | 7 | Rrp8<tm1Jyan> MGI:5635913 | tatgeted mutation 1, Jun Yanagisawa | |||
| loxP | phage P1 loxP | 7 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP site, Saccharomyces cerevisiae FRT (flipase recombination target) site, mouse Rrp8 (Nml)genomic DNA |
| Research application | Cell Biology Research Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 7(3)807-820 (2014). The availability of the BIOLOGICAL RESOURCE is limited to a Recipient of a non- for-profit organization for a not-for-profit research. |
| Depositor | Jun Yanagisawa (University of Tsukuba) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |