Strain Data Sheet

RBRC04605

Strain Information

Image
BRC No.RBRC04605
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Kcnc1-GCaMP2)846Bsi
Former Common namepKv3.1-GCaMP2#846
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Thomas Knoepfel, RIKEN Brain Science Institute. C57BL/6, DBA/2 and ICR mixed background.
Strain descriptionKcnc1-GCaMP2 transgenic mice. Transgene composed of regulatory sequences of rat Kv3.1 (Kcnc1) and the gene for the calcium sensor GCaMP2.
Colony maintenance
References
Activation of cerebellar parallel fibers monitored in transgenic mice expressing a fluorescent Ca2+ indicator protein.
Javier Díez-García, Shinichi Matsushita, Hiroki Mutoh, Junichi Nakai, Masamichi Ohkura, Jennifer Yokoyama, Dimitar Dimitrov, Thomas Knöpfel
Eur. J. Neurosci., 22, 627-635 (2005). 16101744

In vivo calcium imaging from genetically specified target cells in mouse cerebellum.
Diez-Garcia J, Akemann W, Knopfel T
Neuroimage, 34, 859-869 (2007). 17161628

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GCaMP2 RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2 rat Kv3.1 (Kcnc1) promoter
SV40 polyA signalUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat Kv3.1 (Kcnc1) genomic DNA, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Simian virus SV40 polyA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur J. Neurosci., 22, 627-635 (2005). Neuroimage, 34, 859-869 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorThomas Knöepfel (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information

    BRC mice in Publications

    No Data