Strain Data Sheet

RBRC04696

Strain Information

Image
BRC No.RBRC04696
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Agtr1a<tm1Afu>
Former Common nameB6-AT1a KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Akiyoshi Fukamizu, Institute of Applied Biochemistry, Tsukuba Advanced Researrch Alliance, University of Tukuba in 1995.
Strain description
Colony maintenance
References
Angiotensin II type 1a receptor-deficient mice with hypotension and hyperreninemia.
Sugaya T, Nishimatsu S, Tanimoto K, Takimoto E, Yamagishi T, Imamura K, Goto S, Imaizumi K, Hisada Y, Otsuka A, et a l
J. Biol. Chem., 270, 18719-18722 (1995). 7642517

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Agtr1a
MGI:87964		angiotensin II receptor, type 1a13Agtr1a<tm1Afu>
MGI:2156487		targeted mutation 1, Akiyoshi Fukamizu
  • essential hypertension, genetic(MedGEN)

  • renal tubular dysgenesis of genetic origin(MedGEN)
    • lacZ beta-galactosidase (E. coli)13
    neo neomycin resistance gene (E. coli)13 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal drinking behavior(MP:0001422)

  • abnormal kidney interstitium morphology(MP:0011425)

  • abnormal kidney morphology(MP:0002135)

  • decreased systemic arterial systolic blood pressure(MP:0006264)

  • homeostasis/metabolism phenotype(MP:0005376)
    • more 4 phenotypes
  • hypotension(MP:0001596)

  • increased circulating renin level(MP:0003352)

  • increased fluid intake(MP:0011941)

  • increased renin activity(MP:0005582)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse AT1a genomic DNA, E. coli neo<r>, E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter)[update Feb 12, 2014]
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 270, 18719-18722 (1995). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkiyoshi Fukamizu (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Kawakami-Mori F, Nishimoto M, Reheman L, Kawarazaki W, Ayuzawa N, Ueda K, Hirohama D, Kohno D, Oba S, Shimosawa T, Marumo T, Fujita T.
    Aberrant DNA methylation of hypothalamic angiotensin receptor in prenatal programmed hypertension.
    JCI Insight 3(21) (2018) 30385711