Strain Data Sheet

RBRC04714

Strain Information

Image
BRC No.RBRC04714
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Spesp1<tm1Osb>
Former Common nameSpesp1 KO
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strain
Appearance
Strain developmentDeveloped by Drs.Yoshitaka Fujihara and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2006.
Strain description
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
References
Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse.
Fujihara Y, Murakami M, Inoue N, Satouh Y, Kaseda K, Ikawa M, Okabe M
J. Cell Sci., 123, 1531-1536 (2010). 20375058

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site9Frt
Spesp1
MGI:1913962		sperm equatorial segment protein 19Spesp1
loxP phage P1 loxP9loxP
loxP phage P1 loxP9loxP
neo neomycin resistance gene (E. coli)9

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal acrosome morphology(MP:0008898)

  • abnormal sperm physiology(MP:0004543)

  • decreased fertilization frequency(MP:0009647)

  • delayed fertilization(MP:0005411)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Spesp1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter) and polyA signal, P1 phage loxP, yeast FRT (flipase recombination target) site
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 123, 1531-1536 (2010). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Nakata H, Wakayama T, Asano T, Nishiuchi T, Iseki S.
    Identification of sperm equatorial segment protein 1 in the acrosome as the primary binding target of peanut agglutinin (PNA) in the mouse testis.
    Histochem Cell Biol 147(1) 27-38(2017) 27539077