Strain Data Sheet

RBRC04741

Strain Information

Image
BRC No.RBRC04741
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Ltb4r2<tm1Tksh>
Former Common nameB6-BLT2 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Takao Shimizu, Faculty of Medicine, University of Tokyo in 2005. A lacZ-neo was inserted into the protein coding region of Ltb4r2 gene (Expression of lacZ was not detected). The mutant mice were backcrossed to C57BL/6.
Strain description
Colony maintenance
References
Hydroxyeicosanoids bind to and activate the low affinity leukotriene B4 receptor, BLT2.
Yokomizo T, Kato K, Hagiya H, Izumi T, Shimizu T
J. Biol. Chem., 276, 12454-12459 (2001). 11278893

Characterization of a mouse second leukotriene B4 receptor, mBLT2: BLT2-dependent ERK activation and cell migration of primary mouse keratinocytes.
Iizuka Y, Yokomizo T, Terawaki K, Komine M, Tamaki K, Shimizu T
J. Biol. Chem., 280, 24816-24823 (2005). 15866883

12(S)-Hydroxyheptadeca-5Z, 8E, 10E-trienoic acid is a natural ligand for leukotriene B4 receptor 2.
Okuno T, Iizuka Y, Okazaki H, Yokomizo T, Taguchi R, Shimizu T
J. Exp. Med., 205, 759-766 (2008). 18378794

Protective role of the leukotriene B4 receptor BLT2 in murine inflammatory colitis.
Iizuka Y, Okuno T, Saeki K, Uozaki H, Okada S, Misaka T, Sato T, Toh H, Fukayama M, Takeda N, Kita Y, Shimizu T, Nakamura M, Yokomizo T
FASEB J., 24, 4678-4690 (2010). 20667973

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ltb4r2
MGI:1888501		leukotriene B4 receptor 214Ltb4r2<tm1Tksh>
MGI:3785609		targeted mutation 1, Takao Schimizu
lacZ beta-galactosidase (E. coli)14
neo neomycin resistance gene (E. coli)14

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cornea wound healing(MP:0020861)

  • abnormal mast cell physiology(MP:0002423)

  • abnormal sensitization(MP:0011618)

  • decreased susceptibility to type I hypersensitivity reaction(MP:0005597)

  • delayed wound healing(MP:0002908)
    • more 3 phenotypes
  • edema(MP:0001785)

  • increased susceptibility to induced colitis(MP:0008537)

  • increased thigmotaxis(MP:0002797)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse phophoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, E. coli beta-galactosidase, Mouse polyA, Mouse Ltb4r2 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J., 24(12):4678-90 (2010). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakao Shimizu (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data