Strain Data Sheet

RBRC04847

Strain Information

Image
BRC No.RBRC04847
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.B6129-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa>
Former Common nameB6-ROSA/kikGR (floxed) KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura and Osami Kanagawa, Research Center for Allergy and Immunologuy, RIKEN in 2009.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes.
Tomura M, Hata A, Matsuoka S, Shand F H, Nakanishi Y, Ikebuchi R, Ueha S, Tsutsui H, Inaba K, Matsushima K, Miyawaki A, Kabashima K, Watanabe T, Kanagawa O
Sci. Rep., e6030 (2014). 25112380

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sortargeted mutation 1, Osami Kanagawa
kikGRGreen-Red Fluorescent Protein (Favia favus)6kikGRCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Stop, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Favia favus Kikume-Green Red cDNA, chicken poly(A) sequence, mouse Gt(ROSA)26Sor genomic region
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., e6030 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Lab Contact: haruhiko.koseki@riken.jp) using the Approval Form (form D). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing KikGR, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. PLoS One 3 (12): e3944 (2008).
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data