Strain Data Sheet

RBRC04848

Strain Information

Image
BRC No.RBRC04848
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.B6129-Gt(ROSA)26Sor<tm1(CAG-ECFP/Venus)Kgwa>
Former Common nameB6-ROSA/SCAT3.1(floxed) KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura and Osami Kanagawa, Research Center for Allergy and Immunologuy, RIKEN in 2008.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions.
Tomura M, Mori Y S, Watanabe R, Tanaka M, Miyawaki A, Kanagawa O
Int. Immunol., 21, 1145-1150 (2009). 19710208

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ECFP Enhanced Cyan Fluorescent Protein (Aequorea victoria)6ECFP
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-ECFP/Venus)Kgwa>
MGI:4366503		targeted mutation 1, Osami Kanagawa
Venus Yellow Fluorescent Protein (Aequorea victoria)6Venus
loxP phage P1 loxP6loxP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
STOP codon (synthetic)6
Caspase-3 cleavable peptide6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, STOP codon (synthetic), Aequorea victria GFP derivatives (ECFP, Venus) cDNA, Caspase-3 cleavable peptide, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, mouse Gt(ROSA)26Sor region genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 21(10), 1145-1150 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must send the DEPOSITOR a reprint of RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data