Strain Data Sheet

RBRC04874

Strain Information

Image
BRC No.RBRC04874
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP/tDsRed)Utr>/Rbrc
Former Common name
H-2 Haplotype
ES Cell lineB6N-22<UTR> [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center in 2011. B6N-22<Ttr> ES cells were used. C57BL/6N background.
Strain descriptionR26GRR knock-in mice. A construct consisting of the EGFP-tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter, was inserted via homologous recombination into the intron between exons1 and 2 of the ROSA26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrl]
References
Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination.
Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K
Exp. Anim., 62, 295-304 (2013). 24172193

Generation of a Gal4-dependent gene recombination and illuminating mouse.
Masaharu Yoshihara, Teppei Nishino, Naoto Sambe, Takahiro Nayakama, Freddy Radtke, Seiya Mizuno, Satoru Takahashi
Exp. Anim., 71(3), 385-390 (2022). 35444103

Analysis of Notch1 signaling in mammalian sperm development.
Naoto Sambe, Masaharu Yoshihara, Teppei Nishino, Ryosuke Sugiura, Takahiro Nakayama, Chandra Louis, Satoru Takahashi
BMC Res. Notes 16(1) 109 (2023). 37337280

Notch1 signaling is limited in healthy mature kidneys in vivo.
Ryosuke Sugiura, Takahiro Nakayama, Teppei Nishino, Naoto Sambe, Freddy Radtke, Masaharu Yoshihara, Satoru Takahashi
BMC Res. Notes 16(1) 54 (2023). 37069662

Health Report

Examination Date / Room / Rack2025/01/07Room:3-BRack:FSentinel mouse program
2024/10/07Room:3-BRack:FSentinel mouse program
2024/07/08Room:3-BRack:FSentinel mouse program
2024/04/08Room:3-BRack:FSentinel mouse program
2024/01/09Room:3-BRack:FSentinel mouse program
2023/10/10Room:3-BRack:FSentinel mouse program
2023/07/10Room:3-BRack:FSentinel mouse program
2023/04/10Room:3-BRack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
FLAG FLAG tag (synthetic)6FLAG
Frt yeast FRT (flippase recombination target) site6Frt
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA26)Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor<tm1(CAG-EGFP/DsRed)Utr> targeted mutation 1 (CAG-EGFP/DsRed), University of Tsukuba Laboratory Animal Resource Center
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter
tDsRed Red Fluorescent Protein (Discosoma sp.)6tDsRed
SV40 polyA6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNARosa gene (ubiquitous) (mouse), CMV IE enhancer (cytomegalovirus), beta-actin promoter (chicken), beta-globin genomic DNA (rabbit), EGFP (jellyfish), DsRed (red flourescent protein from Discosoma sp.) cDNA, PGK promoter (mouse), neomycin resistance gene (E. coli), polyA additional signal (mouse), FRT sites (yeast), loxP sites (P1 phage), phage T7 gb2 promoter(synthetic), polyA (SV40), FLAG tag (synthetic)
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim 62, 295-304 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: FP license of TBUSA
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Mar 2014, Mouse of the Month Oct 2018

    BRC mice in Publications

    Morikawa K, Nagasaki A, Sun L, Kawase E, Ebihara T, Shirayoshi Y.
    Optogenetic control of early embryos labeling using photoactivatable Cre recombinase 3.0.
    FEBS Open Bio 14(11) 1888-1898(2024) 39223831
    Mizoguchi A, Banno R, Sun R, Yaginuma H, Taki K, Kobayashi T, Sugiyama M, Tsunekawa T, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Suga H, Nagai T, Yamada K, Arima H.
    Glucocorticoid receptor signaling in ventral tegmental area neurons increases the rewarding value of a high-fat diet in mice.
    Sci Rep 11(1) 12873(2021) 34145364
    Hashimoto M, Sasaki H.
    Epiblast Formation by TEAD-YAP-Dependent Expression of Pluripotency Factors and Competitive Elimination of Unspecified Cells.
    Dev Cell 50(2) 139-154.e5(2019) 31204175
    Tsunekawa T, Banno R, Yaginuma H, Taki K, Mizoguchi A, Sugiyama M, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Bettler B, Arima H.
    GABAB Receptor Signaling in the Mesolimbic System Suppresses Binge-like Consumption of a High-Fat Diet.
    iScience 20 337-347(2019) 31610370
    Matsumoto L, Hirota Y, Saito-Fujita T, Takeda N, Tanaka T, Hiraoka T, Akaeda S, Fujita H, Shimizu-Hirota R, Igaue S, Matsuo M, Haraguchi H, Saito-Kanatani M, Fujii T, Osuga Y.
    HIF2α in the uterine stroma permits embryo invasion and luminal epithelium detachment.
    J Clin Invest 128(7) 3186-3197(2018) 29911998
    Shibata M, Banno R, Sugiyama M, Tominaga T, Onoue T, Tsunekawa T, Azuma Y, Hagiwara D, Lu W, Ito Y, Goto M, Suga H, Sugimura Y, Oiso Y, Arima H.
    AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High-Fat Diet.
    Endocrinology 157(4) 1457-66(2016) 26889940
    Hiraoka T, Hirota Y, Saito-Fujita T, Matsuo M, Egashira M, Matsumoto L, Haraguchi H, Dey SK, Furukawa KS, Fujii T, Osuga Y.
    STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation.
    JCI Insight 1(8) (2016) 27358915