Strain Data Sheet

RBRC04878

Strain Information

Image
BRC No.RBRC04878
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;129-Tg(CMV-EGFP,U6-shNanog)1Ttada
Former Common nameNRi-Tg
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Shinpei Yamaguchi and Takashi Tada, Institute for Frontier Medical Sciences, Kyoto University in 2007.
Strain description
Colony maintenanceCarrier x Carrier
References
Cre-lox-regulated conditional RNA interference from transgenes.
Andrea Ventura, Alexander Meissner, Christopher P Dillon, Michael McManus, Phillip A Sharp, Luk Van Parijs, Rudolf Jaenisch, Tyler Jacks
Proc. Natl. Acad. Sci. USA, 13;101(28):10380-5 (2004). 15240889

Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells.
Yamaguchi S, Kurimoto K, Yabuta Y, Sasaki H, Nakatsuji N, Saitou M, Tada T
Development, 136, 4011-4020 (2009). 19906868

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP CMV promoter
Nanog
MGI:1919200		Nanog homeoboxUNNanog mouse U6 promoter
TATAlox phage P1 loxP (modified loxP site)UNTATAlox
TATAlox phage P1 loxP (modified loxP site)UNTATAlox
WPRE Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic lethality, complete penetrance(MP:0011092)

  • increased apoptosis(MP:0006042)
    • Detailed phenotype data

    Ordering Information

    Donor DNA5' LTR, HIV PRE, FLAP, mouse U6 promoter, Phage P1 TATAlox sites (modified loxP site), Cytomegalovirus CMV promoter, jellyfish EGFP cDNA, Nanog shRNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), 3'SIN LTR [update April 12, 2019]
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorTakashi Tada (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Chen CL, Uthaya Kumar DB, Punj V, Xu J, Sher L, Tahara SM, Hess S, Machida K.
    NANOG Metabolically Reprograms Tumor-Initiating Stem-like Cells through Tumorigenic Changes in Oxidative Phosphorylation and Fatty Acid Metabolism.
    Cell Metab 23(1) 206-19(2016) 26724859