Strain Data Sheet
RBRC04885
Strain Information | |
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| Image | |
| BRC No. | RBRC04885 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S4-Cacna1e<tm1Ttan> |
| Former Common name | B6-Cacna1e KO |
| H-2 Haplotype | |
| ES Cell line | J1 [129S4/SvJae] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tsutomu Tanabe, Graduate School of Medicine, Tokyo Medical and Dental University and Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research in 1997. The Cacna1e gene was disrupted by insertion of a lacZ-neo cassette. |
| Strain description | Cacna1e knockout mice. Homozygous and heterozygous mutant mice show reduced responses to somatic inflammatory pain. Heterozygous mutant mice also show reduced respose to visceral inflammatory pain. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Altered pain responses in mice lacking alpha 1E subunit of the voltage-dependent Ca2+ channel. Saegusa H, Kurihara T, Zong S, Minowa O, Kazuno A, Han W, Matsuda Y, Yamanaka H, Osanai M, Noda T, Tanabe T Proc. Natl. Acad. Sci. USA, 97, 6132-6137 (2000). 10801976 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Cacna1e MGI:106217 | calcium channel, voltage-dependent, R type, alpha 1E subunit | 1 | Cacna1e <tm1Ttan> MGI:2179732 | targeted mutation 1, Tsutomu Tanabe | |||
| lacZ | beta-galactosidase (E. coli) | 1 | |||||
| neo | neomycin resistance gene (E. coli) | 1 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, mouse Phosphoglycerate Kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cacna1e genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci USA, 97, 6132-6137 (2000). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE. |
| Depositor | Tsutomu Tanabe (Tokyo Medical and Dental University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Min C, Park J, Kim G, Moon H, Lee SA, Kim D, Moon B, Yang S, Lee J, Kim K, Cho H, Park J, Lee DH, Lee G, Park D. Tim-4 functions as a scavenger receptor for phagocytosis of exogenous particles. Cell Death Dis 11(7) 561(2020) 32703939 |
Parajuli LK, Nakajima C, Kulik A, Matsui K, Schneider T, Shigemoto R, Fukazawa Y. Quantitative regional and ultrastructural localization of the Ca(v)2.3 subunit of R-type calcium channel in mouse brain. J Neurosci 32(39) 13555-67(2012) 23015445 |