Strain Data Sheet

RBRC04892

update : 2025-07-18

Strain Information
Image
BRC No.	RBRC04892
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6(Cg)-Aicda<tm2.1Hon>
Former Common name	B6-AID KI/G23S
H-2 Haplotype
ES Cell line	Bruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain development	Developed by Min Wei and Tasuku Honjo, Gradutate School of Medicine, Kyoto University in 2007.
Strain description
Colony maintenance	Heterozygote x Wild-type [C57BL/6J(Crlj)]
References	Separate domains of AID are required for somatic hypermutation and class-switch recombination. Shinkura R, Ito S, Begum N A, Nagaoka H, Muramatsu M, Kinoshita K, Sakakibara Y, Hijikata H, Honjo T Nat. Immunol., 5, 707-712 (2004). 15195091 Mice carrying a knock-in mutation of Aicda resulting in a defect in somatic hypermutation have impaired gut homeostasis and compromised mucosal defense. Wei M, Shinkura R, Doi Y, Maruya M, Fagarasan S, Honjo T Nat. Immunol., 12, 264-270 (2011). 21258321

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Aicda MGI:1342279	activation-induced cytidine deaminase	6	Aicda<tm2.1Hon> MGI:4942397	targeted mutation 2, Tasuku Honjo			hyper-IgM syndrome type 2(MedGEN)
loxP	phage P1 loxP	6	loxP

Phenotype
Phenotype annotation from literatures by Mammalian phenotype ontology	abnormal gut flora balance (MP:0010377) abnormal somatic hypermutation frequency (MP:0004815) decreased IgG1 level (MP:0008495) immune system phenotype (MP:0005387) increased germinal center B cell number (MP:0008177) more 2 phenotypes increased susceptibility to bacterial infection (MP:0002412) increased susceptibility to bacterial infection induced morbidity/mortality (MP:0009788)
Detailed phenotype data

Ordering Information
Donor DNA	Phage P1 loxP site, mouse Aicda (G23S) genomic DNA
Research application	Cre/loxP system
Specific Term and Conditions	1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Immunol., 12, 264-270 (2011). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Depositor	Tasuku Honjo (Kyoto University)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Honjo Lab HP Genotyping protocol -PCR-

BRC mice in Publications

No Data