Strain Data Sheet

RBRC04916

Strain Information

Image
BRC No.RBRC04916
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gnb1<Gt(prvSStrap)4B8Yiw>
Former Common nameB6-Gnb1 Gt#4B8
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. The mice were backcrossed to C57BL/6 for at least 6 times.
Strain descriptionGbeta1 knockout mice. Homozygous mutant mice are embryonic lethal.
Colony maintenance
References
Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.
Okae H, Iwakura Y
Dev. Dyn., 239, 1089-1101 (2010). 20186915

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CD4 CD4 TM domain sequence (rat)4CD4
Gnb1
MGI:95781		guanine nucleotide binding protein (G protein), beta 14Gnb1<Gt(prvSStrap)4B8Yiw> gene trap 4B8, Yoichiro Iwakura
  • acute lymphoblastic leukemia(MedGEN)

  • intellectual disability, autosomal dominant 42(MedGEN)

  • myelodysplastic syndrome(MedGEN)
    • Hyg hygromycin phasphotransferase (E. coli)4Hyg
    LTR LTR (Mo-MuLV, Mo-MuSV)4LTR
    SA immunoglobulin splice acceptor (mouse)4SA
    lacZ beta-galactosidase (E. coli)4
    polyA TK polyA signal (HSV)4polyA

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal brain morphology(MP:0002152)

  • abnormal cortical ventricular zone morphology(MP:0008458)

  • abnormal embryonic neuroepithelium morphology(MP:0004261)

  • abnormal neural plate morphology(MP:0005657)

  • abnormal neuron differentiation(MP:0009937)
    • more 9 phenotypes
  • abnormal neuronal precursor proliferation(MP:0004948)

  • abnormal suckling behavior(MP:0001436)

  • decreased brain size(MP:0000774)

  • decreased cell proliferation(MP:0000352)

  • exencephaly(MP:0000914)

  • lethality throughout fetal growth and development, incomplete penetrance(MP:0011109)

  • perinatal lethality, incomplete penetrance(MP:0011090)

  • respiratory failure(MP:0001953)

  • thin cerebral cortex(MP:0006254)
    • Detailed phenotype data

    Ordering Information

    Donor DNArabbit beta-globin intron, mouse immunoglobulin splice acceptor (SA), rat CD4 TM domain sequence, E. coli lacZ gene, E. coli hygromycin resistance gene, HSV TK polyA signal, Mo-MuLV, Mo-MuSV LTR
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 239, 1089-1101 (2010).Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data