Strain Data Sheet

RBRC04926

Strain Information

Image
BRC No.RBRC04926
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Epha2<Gt(ROSABetageo)CN3Yiw>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2001. The mice were backcrossed to C57BL/6 for at least 8 times.
Strain descriptionEph receptor 2 knockout mice. Tail abnormality are frequently observed.
Colony maintenance
References
Involvement of EphA2 in the formation of the tail notochord via interaction with ephrinA1.
Naruse-Nakajima C, Asano M, Iwakura Y
Mech. Dev., 102, 95-105 (2001). 11287184

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Epha2
MGI:95278		Eph receptor A24Epha2<Gt(ROSABetageo)CN3Yiw>
MGI:2448472		gene trap CN3, Yoichiro Iwakura
  • cataract 6 multiple types(MedGEN)
    • LTR MoMuLV long terminal repeat4LTR
    SA Splice acceptor4SA
    lacZ beta-galactosidase (E. coli)4
    neo neomycin resistance gene (E. coli)4
    nls SV40 large T antigen nuclear localization signal (NLS)4nls
    poly A polyadenylic acid additional signal4poly A

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal axial mesoderm morphology(MP:0001684)

  • abnormal caudal vertebrae morphology(MP:0002759)

  • abnormal neural tube morphology(MP:0002151)

  • abnormal sclerotome morphology(MP:0006029)

  • abnormal tail morphology(MP:0002111)
    • more 5 phenotypes
  • abnormal vascular development(MP:0000259)

  • cataract(MP:0001304)

  • kinked tail(MP:0000585)

  • short tail(MP:0000592)

  • split notochord(MP:0004713)
    • Detailed phenotype data

    Ordering Information

    Donor DNALTR (Mo-MuLV), Splice acceptor, Simian virus 40 Large T antigen nuclear localization signal, E. coli beta geo (Neomycin resistance gene, lacZ), polyadenylic acid additional signal, mouse Epha2 genomic DNA [update Feb 28, 2014]
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech. Dev., 102, 95-105 (2001). Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data