Strain Data Sheet

RBRC04950

Strain Information

Image
BRC No.RBRC04950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Traf6<tm1Jino>
Former Common nameB6-Traf6 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentA 2.5 kb genomic fragement was replaced with a neomycin selection cassette. The mutant mice were crossed with C57BL/6.
Strain descriptionTraf6 knockout mice. A 2.5 kb genomic fragment was replaced with a neomycin selection cassette. Homozygous mutant mice exhibit severe osteopetrosis and abnormal immune cell development and function, and die within around 2 weeks after birth. Homozygous mice represented only 12% of offspring. C57BL/6 background (RBRC04950), BALB/c background (RBRC05386).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Severe osteopetrosis, defective interleukin-1 signalling and lymph node organogenesis in TRAF6-deficient mice.
Naito A, Azuma S, Tanaka S, Miyazaki T, Takaki S, Takatsu K, Nakao K, Nakamura K, Katsuki M, Yamamoto T, Inoue J
Genes Cells., 4, 353-362 (1999). 10421844

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Traf6
MGI:108072		TNF receptor-associated factor 62Traf6<tm1Jino>
MGI:2675469		targeted mutation 1, Jun-ichiro Inoue
neo neomycin resistance gene (E. coli)2 mouse Polr2a promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Meibomian gland development(MP:0013385)

  • abnormal Meibomian gland morphology(MP:0005252)

  • abnormal coat/ hair morphology(MP:0000367)

  • abnormal hair follicle development(MP:0003704)

  • abnormal male preputial gland morphology(MP:0002717)
    • more 20 phenotypes
  • abnormal osteoclast differentiation(MP:0008396)

  • abnormal sebaceous gland morphology(MP:0000647)

  • abnormal skin pigmentation(MP:0002095)

  • abnormal sweat gland morphology(MP:0000674)

  • absent eccrine glands(MP:0000577)

  • absent guard hair(MP:0006365)

  • alopecia(MP:0000414)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased immature B cell number(MP:0008215)

  • decreased lymph node number(MP:0002219)

  • failure of tooth eruption(MP:0000121)

  • kinked tail(MP:0000585)

  • osteopetrosis(MP:0000067)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, complete penetrance(MP:0011085)

  • preweaning lethality, incomplete penetrance(MP:0011110)

  • small perianal sebaceous gland(MP:0013382)

  • small sebaceous gland(MP:0012725)

  • translucent skin(MP:0001201)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Polr2a promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Traf6 genomic DNA
    Research application
    Specific Term and Conditions1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    2. RECIPIENT must contact the DEPOSITOR in the case of application for any patents,any manuscripts, any public presentation,or commercial use, based on the results from the use of the BIOLOGICAL RESOURCE.
    3. RECIPIENT must not yield the BIOLOGICAL RESOURCE to other parties or persons.
    DepositorJunichiro Inoue (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2012

    BRC mice in Publications

    Ismail HM, Didangelos A, Vincent TL, Saklatvala J.
    Rapid Activation of Transforming Growth Factor β-Activated Kinase 1 in Chondrocytes by Phosphorylation and K63 -Linked Polyubiquitination Upon Injury to Animal Articular Cartilage.
    Arthritis Rheumatol 69(3) 565-575(2017) 27768832