Strain Data Sheet

RBRC05065

Strain Information

Image
BRC No.RBRC05065
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Sox1<tm1(cre)Take>
Former Common nameB6-Sox1/cre KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentCre recombinase was inserted into the ATG codon start site if Sox1 by in-frame. PGK-neo cassette was removed by Flp recombinase expression.
Strain descriptionSox1 cre knock-in mice. knocked into the Sox1 gene. No obvious defect at the heterozygous state which is used for the conditional KO or cell tracing purposes.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
References
Neuroepithelial cells supply an initial transient wave of MSC differentiation.
Takashima Y, Era T, Nakao K, Kondo S, Kasuga M, Smith A G, Nishikawa S
Cell, 129, 1377-1388 (2007). 17604725

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:BSentinel mouse program
2024/10/21Room:3-CRack:BSentinel mouse program
2024/07/22Room:3-CRack:BSentinel mouse program
2024/04/22Room:3-CRack:BSentinel mouse program
2024/01/22Room:3-CRack:BSentinel mouse program
2023/10/23Room:3-CRack:BSentinel mouse program
2023/07/24Room:3-CRack:BSentinel mouse program
2023/05/22Room:3-CRack:BAdditional tests
2023/04/24Room:3-CRack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site8Frt
Sox1
MGI:98357
SRY (sex determining region Y)-box 18Sox1<tm1(cre)Take>
MGI:3807952
targeted mutation 1, Takumi Era
cre Phage P1 Cre recombinase8cre

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 cre recombinase, yeast FRT (flippase recombination target) site, mouse Sox1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0525K, and Web Site (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Cell, 129, 1377-1388 (2007).)
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Koizumi M, Eto H, Saeki M, Seki M, Fukushima T, Mukai S, Ide H, Sera Y, Iwasaki M, Suzuki Y, Tohei A, Kishi Y, Honda H.
    UTX deficiency in neural stem/progenitor cells results in impaired neural development, fetal ventriculomegaly, and postnatal death.
    FASEB J 36(12) e22662(2022) 36412518
    Sakai D, Sugawara T, Kurokawa T, Murakami Y, Tomosugi M, Masuta H, Sakata-Haga H, Hatta T, Shoji H.
    Hif1α-dependent hypoxia signaling contributes to the survival of deep-layer neurons and cortex formation in a mouse model.
    Mol Brain 15(1) 28(2022) 35361248