Strain Data Sheet

RBRC05075

Strain Information

Image
BRC No.RBRC05075
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(CAG-flag-macroH2A1.2)
Former Common nameB6-Flag/mH2A1.2 Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nashun Buhe, Masashi Yukawa and Fugaku Aoki, Graduate School of Frontier Sciences, The University of Tokyo in 2009. Transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionFlag-macroH2A1.2 (H2afy) transgenic mice.
Colony maintenanceCarrier x Noncarrier
References
Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice.
Nashun B, Yukawa M, Liu H, Akiyama T, Aoki F
Development, 137, 3785-3794 (2010). 20943707

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG
H2afy H2A histone family, member YUNH2afy CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAFlag-tag cDNA, Mouse Histone macroH2A1.2 (H2afy) gene cDNA, CMV IE enhancer, Chicken CAG prompter (beta-actin promoter + globin intron), Rabbit beta-globin PolyA signal
    Research application
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorFugaku Aoki (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Mouse of the Month Oct 2011

    BRC mice in Publications

    No Data