Strain Data Sheet

RBRC05155

Strain Information

Image
BRC No.RBRC05155
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-EGFP/TeNT)Imayo>
Former Common nameR26-CAG-FRT-EGFP-TeNT
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. loxP-Stop-loxP cassette was removed by crossing with TNAP-Cre mice (C57BL/6).
Strain descriptionEGFP-TeNTpest is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. EGFP-TeNTpest is expressed under CAG promoter.
Colony maintenance
References
Continuous postnatal neurogenesis contributes to formation of the olfactory bulb neural circuits and flexible olfactory associative learning.
Sakamoto M, Ieki N, Miyoshi G, Mochimaru D, Miyachi H, Imura T, Yamaguchi M, Fishell G, Mori K, Kageyama R, Imayoshi I
J. Neurosci., 34, 5788-5799 (2014). 24760839

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.2(CAG-EGFP/TeNT)Imayo> targeted mutation 1.2, Itaru Imayoshi
Odc1 mouse ornithine decarboxylase (PEST sequence) cDNA6Odc1
TeNT tetanus neurotoxin light chain (Clostridium tetani)6TeNT
loxP phage P1 loxP6loxP
poly A polyA6poly A
puro puromycin acyltransferase (Streptomyces alboniger)6puro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAsplice acceptor, S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP site, Yeast FRT (flippase recombination target) site, Jellyfish EGFP cDNA, Clostridium tetani TeNT light chain, mouse PEST sequence, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The Journal of Neuroscience, 34, 5788-5799 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data