Strain Information | |
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Image | |
BRC No. | RBRC05163 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-GCaMP3)Imayo> |
Former Common name | R26-CAG-LoxP-GCaMP3 |
H-2 Haplotype | |
ES Cell line | TC1 [129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6). |
Strain description | GCaMP3 is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, GCaMP3 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. |
Colony maintenance | |
References | 3D clustering of GABAergic neurons enhances inhibitory actions on excitatory neurons in the mouse visual cortex. Ebina T, Sohya K, Imayoshi I, Yin S T, Kimura R, Yanagawa Y, Kameda H, Hioki H, Kaneko T, Tsumoto T Cell Rep., 9(5):1896-1907 (2014). 25464846 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
HSV thymidine kinase polyA signal | 6 | ||||||
SV40 late polyA signal | 6 | ||||||
Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
GCaMP3 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | 6 | GCaMP3 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
GH | growth hormone polyadenylation signal | 6 | GH | ||||
Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor | targeted mutation 1.1, Itaru Imayoshi | |||
SA | splice acceptor sequence | 6 | SA | ||||
WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) pA | 6 | WPRE | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
puro | puromycin acyltransferase (Streptomyces alboniger) | 6 | puro |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | SA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, phage P1 loxP sites, Yeast FRT (flippase recombination target) site, GCaMP3(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE) |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 9(5):1896-1907 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. |
Depositor | Itaru Imayoshi (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Murata T, Honda T, Egawa G, Yamamoto Y, Ichijo R, Toyoshima F, Dainichi T, Kabashima K. Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification. Sci Rep 8(1) 6610(2018) 29700333 |