Strain Data Sheet

RBRC05165

Strain Information

Image
BRC No.RBRC05165
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Slc17a7<tm1.1(IRES-EGFP/TeNT)Imayo>
Former Common nameVGLUT1-LoxP-Stop-LoxP-IRES2-EGFP-TeNT
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6).
Strain descriptionEGFP-TeNT is expressed after Cre-recombination in VGLUT1-expressing cells. LoxP-Stop-LoxP-IRES2-EGFP-TeNT sequence is knocked into 3’UTR region of VGLUT1 gene.
Colony maintenance
References
Continuous postnatal neurogenesis contributes to formation of the olfactory bulb neural circuits and flexible olfactory associative learning.
Sakamoto M, Ieki N, Miyoshi G, Mochimaru D, Miyachi H, Imura T, Yamaguchi M, Fishell G, Mori K, Kageyama R, Imayoshi I
J. Neurosci., 34, 5788-5799 (2014). 24760839

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)7EGFP
Frt yeast FRT (flippase recombination target) site7Frt
Ires internal ribosomal entry site (EMCV)7
Slc17a7
MGI:1920211		solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 77Slc17a7<tm1.1(IRES-EGFP/TeNT)Imayo> targeted mutation 1.1, Itaru Imayoshi
TeNT tetanus neurotoxin light chain (Clostridium tetani)7TeNT
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP
SV40 early polyA signal7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, SV40 early polyA signal, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP cDNA, Clostridium tetani TeNT light chain, Yeast FRT (flippase recombination target) site
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 34, 5788-5799 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data