Strain Data Sheet

RBRC05276

Strain Information

Image
BRC No.RBRC05276
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameKIF-k (Acc. No.CDB0440K)
Former Common nameKIF-k (Acc. No.CDB0440K)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ryuichi Nishinakamura, Institute of Molecular Embryology and Genetics, Kumamoto University.
Strain descriptionKif26b, kinesin family member 26B knockout mice (KIF-k(lacZ)). Homozygous mice die within 24hrs after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme.
Uchiyama Y, Sakaguchi M, Terabayashi T, Inenaga T, Inoue S, Kobayashi C, Oshima N, Kiyonari H, Nakagata N, Sato Y, Sekiguchi K, Miki H, Araki E, Fujimura S, Tanaka S S, Nishinakamura R
Proc. Natl. Acad. Sci. USA, 107, 9240-9245 (2010). 20439720

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site1Frt
Frt yeast FRT (flippase recombination target) site1Frt
Kif26b
MGI:2447076		kinesin family member 26B1Kif26b <tm1Ryn> targeted mutation 1, Ryuichi Nishinakamura
lacZ beta-galactosidase (E. coli)1
lox71 mutant lox sequence1lox71
loxP phage P1 loxP1loxP
neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal kidney cortex morphology(MP:0000521)

  • abnormal kidney development(MP:0000527)

  • absent kidney(MP:0000520)

  • impaired branching involved in ureteric bud morphogenesis(MP:0004936)

  • kidney degeneration(MP:0009641)
    • more 3 phenotypes
  • neonatal lethality(MP:0002058)

  • single kidney(MP:0003604)

  • small kidney(MP:0002989)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 lox71 site, E. coli lacZ, polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK3’end, phage P1 loxP site, mouse Kif26b genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Ryuichi Nishinakamura, Kumamoto University, E-mail: ryuichi@gpo.kumamoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0440K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A. 2010 107(20):9240-5.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data