Strain Information | |
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Image | |
BRC No. | RBRC05289 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | D2;B6-Tg(CAG-EGFP)C14-Y01-FM131Osb |
Former Common name | D2-CAG-GFP#131 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kei Ishii, Graduate School of Medicine, Kyoto University. C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice were backcrossed to DBA/2 (N5, Oct 2011) |
Strain description | This transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. DBA/2 background. |
Colony maintenance | Carrier x Noncarrier [or Crossing to DBA/2CrSlc] |
References | Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9. Sato T, Sakuma T, Yokonishi T, Katagiri K, Kamimura S, Ogonuki N, Ogura A, Yamamoto T, Ogawa T Stem Cell Rep., (2015). 26095606Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, Shinohara T Biol. Reprod., 69(2):612-616 (2003). 12700182 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GFP | Green Fluorescent Protein (Aequorea victoria) | 14 | transgene insertion 131, Research Institute for Microbial Diseases, Osaka University | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 69(2):612-616 (2003). Stem Cell Rep., (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must make MTA specified for Green Mouse with Prof. Masaru Okabe, Osaka University prior to obtaining the BIOLOGICAL RESOURCE. |
Depositor | Takashi Shinohara (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |