Strain Data Sheet

RBRC05290

Strain Information

Image
BRC No.RBRC05290
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Dscam<tm1.1Kzy>/A151Kzy
Former Common nameB6-Dscam KO#A151
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentExon 1 was removed by crossing with CAG-cre (B6 background). The mice were backcrossed to C57BL/6 over 21 times.
Strain descriptionDscam knockout mice. Dscam, Down syndrome cell adhesion molecule is a gene of neural Ig superfamily mediates cell adhesion and axon outgrowth. Homozygous mutant mice of C57BL/6 genetic background die within 24 hours after birth due to central respiratory defects. In BALB/c mixed background, Homozygous mutant mice are viable and survive to adulthood.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death.
Amano K, Fujii M, Arata S, Tojima T, Ogawa M, Morita N, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, Korenberg J R, Arata A, Yamakawa K
J. Neurosci., 29, 2984-2996 (2009). 19261893

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dscam
MGI:1196281		DS cell adhesion molecule16Dscam<tm1.1Kzy>
MGI:3840666		targeted mutation 1.1, Kazuhiro Yamakawa
loxP phage P1 loxP16loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal breathing pattern(MP:0001951)

  • abnormal central pattern generator function(MP:0003880)

  • abnormal respiration(MP:0001943)

  • abnormal somatic nervous system physiology(MP:0005423)

  • apnea(MP:0001957)
    • more 7 phenotypes
  • decreased pulmonary respiratory rate(MP:0005574)

  • delayed parturition(MP:0012008)

  • increased neuron number(MP:0008947)

  • mortality/aging(MP:0010768)

  • neonatal lethality, incomplete penetrance(MP:0011088)

  • postnatal lethality, complete penetrance(MP:0011085)

  • respiratory distress(MP:0001954)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP site, mouse Dscam genomic DNA
    Research applicationCre/loxP system
    Mouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 29, 2984-2996 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuhiro Yamakawa (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2012

    BRC mice in Publications

    Lim CS, Kim MJ, Choi JE, Islam MA, Lee YK, Xiong Y, Shim KW, Yang JE, Lee RU, Lee J, Park P, Kwak JH, Seo H, Kim CH, Lee JH, Lee YS, Hwang SK, Lee K, Lee JA, Kaang BK.
    Dysfunction of NMDA receptors in neuronal models of an autism spectrum disorder patient with a DSCAM mutation and in Dscam-knockout mice.
    Mol Psychiatry 26(12) 7538-7549(2021) 34253863