Strain Data Sheet
RBRC05323
Strain Information | |
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| Image | |
| BRC No. | RBRC05323 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | 129-Sirt1<tm1Mcby> |
| Former Common name | STOCK Sirt1<tm1Mcby> |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A hygromycin resistance cassette was inserted to replace exons 5 and 6. 129 background. |
| Strain description | SirT1 knockout mice. A hygromycin resistance cassette was inserted to replace exons 5 and 6 of Sirt1 gene. Homozygous mutant mice die at birth. When outbred to CD1, SirT1 KO mice have short snouts, eyelid defect and are smaller than littermates at weaning. |
| Colony maintenance | Heterozygote X Wild-type |
| References | The mammalian SIR2alpha protein has a role in embryogenesis and gametogenesis. McBurney M W, Yang X, Jardine K, Hixon M, Boekelheide K, Webb J R, Lansdorp P M, Lemieux M Mol. Cell. Biol., 23, 38-54 (2003). 12482959 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Hyg | hygromycin phasphotransferase (E. coli) | 10 | Hyg | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | |||
| IRES | internal ribosomal entry site (EMCV) | 10 | |||||
| Sirt1 MGI:2135607 | sirtuin 1 | 10 | Sirt1<tm1Mcby> MGI:2448246 | targeted mutation 1, Michael W McBurney | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 72 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Pgk1 splice acceptor (SA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Hygromycin resistance gene, mouse Pgk1 splice donor (SD), mouse Sirt1 genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Michael W. McBurney (Ottawa Hospital Research Institute) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kumar A, Daitsh Y, Ben-Aderet L, Qiq O, Elayyan J, Batshon G, Reich E, Maatuf YH, Engel S, Dvir-Ginzberg M. A predicted unstructured C-terminal loop domain in SIRT1 is required for cathepsin B cleavage. J Cell Sci 131(16) (2018) 30054388 |
Tomita T, Hamazaki J, Hirayama S, McBurney MW, Yashiroda H, Murata S. Sirt1-deficiency causes defective protein quality control. Sci Rep 5 12613(2015) 26219988 |