Strain Data Sheet
RBRC05384
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC05384 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(Prdm14-Venus)1Sait/SaitRbrc |
| Former Common name | Prdm14-mVenus (Acc. No. CDB0461T) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6. |
| Strain description | This transgenic strain expresses a plasma membrane anchored Venus (mVenus) under the control of the mouse Prdm14 promoter. |
| Colony maintenance | Carrier x Nocarrier [C57BL/6N] |
| References | Critical function of Prdm14 for the establishment of the germ cell lineage in mice. Yamaji M, Seki Y, Kurimoto K, Yabuta Y, Yuasa M, Shigeta M, Yamanaka K, Ohinata Y, Saitou M Nat. Genet., 40, 1016-1022 (2008). 18622394 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GH | Growth hormone polyA (Bovine) | UN | GH | ||||
| Prdm14 | PR domain containing 14 | UN | Prdm14 | ||||
| Tg(Prdm14-Venus)1Sait | UN | Tg(Prdm14-Venus)1Sait MGI:3805974 | transgene insertion 1, Mitinori Saitou | ||||
| mVenus | Yellow Fluorescent Protein (Aequorea victoria) | UN | mVenus | ||||
| mouse Igk leader sequence | UN | ||||||
| chimeric intron of the pCL neo vector | UN | ||||||
| PDGF receptor anchoring domain | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | chimeric intron of the pCL neo vector, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus), mouse Igk leader sequence, PDGF receptor anchoring domain, Bovine growth hormone polyadenylation signal sequence, mouse Prdm14 genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0461T and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat Genet. 2008 40(8):1016-22.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. (5) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | RIKEN CDB (RIKEN CDB) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Aug 2013 |
BRC mice in Publications |
|---|
Grosswendt S, Kretzmer H, Smith ZD, Kumar AS, Hetzel S, Wittler L, Klages S, Timmermann B, Mukherji S, Meissner A. Epigenetic regulator function through mouse gastrulation. Nature 584(7819) 102-108(2020) 32728215 |