Strain Data Sheet

RBRC05427

Strain Information

Image
BRC No.RBRC05427
TypeTransgeneCartagena
SpeciesMus musculus
Strain name129.B6C3-Tg(Dkk3-cre)D9Tfur
Former Common name129-Dkk3-cre Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2007. The transgene was injected into the B6C3F1 fertilized eggs. The transgenic mice were backcrossed to 126S6 mice over 8 generations.
Strain descriptionThis transgenic mouse expresses Cre recombinase gene under the control of the 12-kb mouse Dkk3 promoter. Cre activity is detectable from embryonic day 10.5 (E10.5) and generally restricted to the retina during embryogenesis.
Colony maintenanceCarrier x Noncarrier [or Crossing to 129S6/SvEvTac]
References
Dkk3-Cre BAC transgenic mouse line: a tool for highly efficient gene deletion in retinal progenitor cells.
Sato S, Inoue T, Terada K, Matsuo I, Aizawa S, Tano Y, Fujikado T, Furukawa T
Genesis, 45, 502-507 (2007). 17661397

Health Report

Examination Date / Room / Rack2023/09/11Room:3-3Rack:BSentinel mouse program
2023/06/13Room:3-3Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre<Tg(Dkk3-cre)D9Tfur>
MGI:4881674		transgene insertion D9, Takahisa Furukawamouse DKK3 promoter
loxP phage P1 loxPUNloxP
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNABAC clone RP23-12M6 containing mouse DKK3 genomic DNA, P1 Phage Cre recombinase, SV40 PolyA signal, Phage P1 loxP derived from pBACe3.6 vector
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis. 45, 502-507 (2007).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data