Strain Data Sheet

RBRC05428

Strain Information

Image
BRC No.RBRC05428
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Engase<tm1Tasuz>
Former Common name
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Tadashi Suzuki, RIKEN Advanced Science Institute. A PGK-neo selection cassette was transferred into 129 ES cells to replace Exon 5 of Engase gene. The mutant mice were backcrossed C57BL/6 over 6 times.
Strain descriptionEngase knockout mice. Exon 5 of Engase gene was replaced with a PGK-neo selection cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenance
References
Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells.
Huang C, Harada Y, Hosomi A, Masahara-Negishi Y, Seino J, Fujihira H, Funakoshi Y, Suzuki T, Dohmae N, Suzuki T
Proc. Natl. Acad. Sci. USA, 112(5) 1398-403 (2015). 25605922

Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.
Fujihira H, Masahara-Negishi Y, Tamura M, Huang C, Harada Y, Wakana S, Takakura D, Kawasaki N, Taniguchi N, Kondoh G, Yamashita T, Funakoshi Y, Suzuki T
PLoS Genet., 13(4):e1006696 (2017). 28426790

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Engase
MGI:2443788		endo-beta-N-acetylglucosaminidase11Engase<tm1Tasuz> targeted mutation 1, Tadashi Suzuki
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • cellular phenotype(MP:0005384)

  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk1 poly A, mouse Engase genomic DNA [The SV40 Poly A signal was not detected by PCR.]
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 13(4):e1006696 (2017).
    DepositorTadashi Suzuki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data