Strain Data Sheet

RBRC05429

Strain Information

Image
BRC No.RBRC05429
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBXSB.B6-Fcer1g<tm1Tks>/Hros
Former Common nameBXSB-Fcer1g KO BXSB. gamma-/-
H-2 Haplotype
ES Cell lineBL/6-III [C57BL/6J]
Background strain
Appearance
Strain developmentDeveloped by Sachiko Hirose, Department of Internal Medicine, Juntendo University School of Medicine in 2006. A neomycin selection cassette was transferred into BL/6-III ES cells to delete and replace exon 2 of Fcer1g gene. The mutant mice were backcrossed BXSB strain over 20 generations.
Strain descriptionIgG Fc receptor common gamma knockout mice. BXSB background. Exon 2 of Fcer1g gene was deleted and replaced with a neomycin selection cassette. Autoimmune disease features of male were suppressed in homozygous mutant mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to BXSB/MpJJmsSlc (female)]
References
Resistance of Fc receptor- deficient mice to fatal glomerulonephritis.
Park S Y, Ueda S, Ohno H, Hamano Y, Tanaka M, Shiratori T, Yamazaki T, Arase H, Arase N, Karasawa A, Sato S, Ledermann B, Kondo Y, Okumura K, Ra C, Saito T
J. Clin. Invest., 102, 1229-1238 (1998). 9739057

Genetic dissection of the effects of stimulatory and inhibitory IgG Fc receptors on murine lupus.
Lin Q, Xiu Y, Jiang Y, Tsurui H, Nakamura K, Kodera S, Ohtsuji M, Ohtsuji N, Shiroiwa W, Tsukamoto K, Amano H, Amano E, Kinoshita K, Sudo K, Nishimura H, Izui S, Shirai T, Hirose S
J. Immunol., 177, 1646-1654 (2006). 16849473

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fcer1g
MGI:95496		Fc receptor, IgE, high affinity I, gamma polypeptide1Fcer1g<tm1Tks>
MGI:2384004		targeted mutation 1, Takashi Saito
neo neomycin resistance gene (E. coli)1 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal CD4-positive, alpha-beta T cell physiology(MP:0005463)

  • abnormal NK T cell physiology(MP:0008042)

  • abnormal NK cell physiology(MP:0010766)

  • abnormal antigen presentation(MP:0001835)

  • abnormal chemokine secretion(MP:0008722)
    • more 21 phenotypes
  • abnormal interferon-gamma secretion(MP:0008559)

  • abnormal interleukin-17 secretion(MP:0008680)

  • abnormal macrophage physiology(MP:0002451)

  • decreased IgG level(MP:0001805)

  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • decreased T cell proliferation(MP:0005095)

  • decreased dendritic cell number(MP:0008127)

  • decreased inflammatory response(MP:0001876)

  • decreased interferon-gamma secretion(MP:0008567)

  • decreased interleukin-2 secretion(MP:0008688)

  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • decreased susceptibility to autoimmune hemolytic anemia(MP:0004828)

  • decreased susceptibility to induced morbidity/mortality(MP:0009764)

  • decreased susceptibility to type IV hypersensitivity reaction(MP:0005616)

  • decreased susceptibility to xenobiotic induced morbidity/mortality(MP:0009767)

  • decreased tumor necrosis factor secretion(MP:0008561)

  • impaired macrophage phagocytosis(MP:0001798)

  • impaired natural killer cell mediated cytotoxicity(MP:0005070)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, mouse fcer1g genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 177, 1646-1654 (2006).
    DepositorSachiko Hirose (Juntendo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data