Strain Data Sheet

RBRC05432

Strain Information

Image
BRC No.RBRC05432
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-D1Pas1<tm1(KOMP)Vlcg>/D4
Former Common nameB6-D1Pas1 KO#D4
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
D1Pas1
MGI:91842
DNA segment, Chr 1, Pasteur Institute 11D1Pas1<tm1(KOMP)Vlcg>
MGI:4399403
targeted mutation 1, Velocigene
lacZ beta-galactosidase (E. coli)1
loxP phage P1 loxP1loxP
loxP phage P1 loxP1loxP
neo neomycin resistance gene (E. coli)1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • male infertility(MP:0001925)

  • small testis(MP:0001147)
  • Detailed phenotype data

    Ordering Information

    Donor DNAVelocigene cassette ZEN-Ub1(E. coli lacZ, SV40 polyA, Phage P1 loxP sites, human ubiquitin C promoter, synthetic bacterial EM7 promoter (T7 phage), E. coli neo, mouse Pgk1 polyA), mouse D1Pas1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ogonuki N, Kyogoku H, Hino T, Osawa Y, Fujiwara Y, Inoue K, Kunieda T, Mizuno S, Tateno H, Sugiyama F, Kitajima TS, Ogura A.
    Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes.
    EMBO Rep 23(7) e54992(2022) 35587095
    Inoue H, Ogonuki N, Hirose M, Hatanaka Y, Matoba S, Chuma S, Kobayashi K, Wakana S, Noguchi J, Inoue K, Tanemura K, Ogura A.
    Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.
    Biochem Biophys Res Commun 478(2) 592-8(2016) 27473657