Strain Data Sheet

RBRC05437

Strain Information

Image
BRC No.RBRC05437
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Fad104<tm1Maim>/3Maim
Former Common name
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Graduate School of Pharmaceutical Sciences, Nagoya City University and Genome Information Research Center, Osaka University in 2006. C57BL/6J background. Main line (RBRC05437, line 3), Subline (RBRC05438, line 9).
Strain descriptionFad104, factor for adipocyte differentiation 104 (Fndc3b, fibronectin type III domain containing 3B) gene knockout mice. Fad104 is a protein containing 9 repeats of the fibronectin type III domain and a transmembrane domain that transiently expresses at the early stage of adipocyte differentiation. Homozygous mutant mice are die within 1 day after birth. Analyses of MEFs from mutant mice revealed that disruption of this gene caused a reduction in the ability to differentiate, proliferate, adhere, spread and migrate. Heterozygous mutant mice show a less body weight gain than wild-types. Exon 2 was replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote X Wild-type [C57BL/6J(Crlj)]
References
The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis.
Tominaga K, Kondo C, Johmura Y, Nishizuka M, Imagawa M
FEBS Lett., 577, 49-54 (2004). 15527760

Disruption of the novel gene fad104 causes rapid postnatal death and attenuation of cell proliferation, adhesion, spreading and migration.
Nishizuka M, Kishimoto K, Kato A, Ikawa M, Okabe M, Sato R, Niida H, Nakanishi M, Osada S, Imagawa M
Exp. Cell. Res., 315, 809-819 (2009). 19138685

Fad104, a positive regulator of adipogenesis, negatively regulates osteoblast differentiation.
Kishimoto K, Kato A, Osada S, Nishizuka M, Imagawa M
Biochem. Biophys. Res. Commun., 397, 187-191 (2010). 20493170

Indispensable role of factor for adipocyte differentiation 104 (fad104) in lung maturation.
Kishimoto K, Nishizuka M, Ueda T, Kajita K, Ugawa S, Shimada S, Osada S, Imagawa M
Exp. Cell. Res., 317, 2110-2123 (2011). 21704616

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fndc3b
MGI:1919257		fibronectin type III domain containing 3B3Fndc3b<tm1Maim>
MGI:4822558		targeted mutation 1, Masayoshi Imagawa

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell adhesion(MP:0003566)

  • abnormal cell differentiation(MP:0005076)

  • decreased fibroblast cell migration(MP:0011708)

  • decreased fibroblast proliferation(MP:0011704)

  • homeostasis/metabolism phenotype(MP:0005376)
    • more 2 phenotypes
  • increased bone ossification(MP:0020039)

  • neonatal lethality, complete penetrance(MP:0011087)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomysin resistance gene, mouse PGK promoter, HSV HSV-tk, mouse PGK polyA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Cell. Res., 315, 809-819 (2009). Exp. Cell. Res., 317, 2110-2123 (2011). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr.Makoto Nishizuka (nishizuka@hirosaki-u.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayoshi Imagawa (Nagoya City University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data