Strain Data Sheet

RBRC05439

Strain Information

Image
BRC No.RBRC05439
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Muc21<tm2(Venus)Tats>/1
Former Common nameC57BL/6J-Muc21<tm2(Venus)Tats>/1TatsRbrc
H-2 Haplotype
ES Cell lineB6J-S1<UTR> [C57BL/6J]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tatsuro Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2011. C57BL/6J background. Main line (RBRC05439), Subline (RBRC05440).
Strain descriptionMucin 21/epiglycanin venus knock-in mice. A PGK-neo cassette and venus gene was inserted into Muc21 gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJJcl]
References
Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin.
Itoh Y, Kamata-Sakurai M, Denda-Nagai K, Nagai S, Tsuiji M, Ishii-Schrade K, Okada K, Goto A, Fukayama M, Irimura T
Glycobiology, 18, 74-83 (2008). 17977904

Mucin 21/epiglycanin modulates cell adhesion.
Yi Y, Kamata-Sakurai M, Denda-Nagai K, Itoh T, Okada K, Ishii-Schrade K, Iguchi A, Sugiura D, Irimura T
J. Biol. Chem., 285, 21233-21240 (2010). 20388707

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site17Frt
Muc21
MGI:3779983		mucin 2117Muc21<tm2(Venus)Tats>
MGI:7493237		targeted mutation 2, Tatsuro Irimura
Venus Yellow Fluorescent Protein (Aequorea victoria)17Venus
loxP phage P1 loxP17loxP
neo neomycin resistance gene (E. coli)17

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Muc21, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, PhageP1 loxP, Yeast FRT, Aequorea victoria Venus
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTatsuro Irimura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data