Strain Data Sheet

RBRC05445

Strain Information

Image
BRC No.RBRC05445
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Htr5b<tm1(tTA)Rhn>
Former Common nameHtr5B-tTA KI, B6-Htr5b/tTA KI
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. The mutant mice were crossed to C57BL/6J over 6 times.
Strain descriptionHtr5B-tTA knock-in mice. The tetracycline-controlled transactivator (tTA) gene was inserted into the Htr5b gene. tTA expression is detected in medial habenular and inferior olivary nuclei, and hippocampus CA1 region.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Activation of ventral CA1 hippocampal neurons projecting to the lateral septum during feeding.
Kosugi K, Yoshida K, Suzuki T, Kobayashi K, Yoshida K, Mimura M, Tanaka K F
Hippocampus 33296119

Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system.
Tanaka K F, Matsui K, Sasaki T, Sano H, Sugio S, Fan K, Hen R, Nakai J, Yanagawa Y, Hasuwa H, Okabe M, Deisseroth K, Ikenaka K, Yamanaka A
Cell Rep., 2(2):397-406 (2012). 22854021

Serotonin-mediated inhibition of ventral hippocampus is required for sustained goal-directed behavior.
Yoshida K, Drew M R, Mimura M, Tanaka K F
Nat. Neurosci., 22(5):770-777 (2019). 30988523

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin polyA1HBB2
Htr5b
MGI:96284		5-hydroxytryptamine (serotonin) receptor 5B1Htr5b <tm1(tTA)Rhn> targeted mutation 1, Rene Hen
loxP phage P1 loxP1loxP
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)1tTA

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAtTA(E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), rabbit beta-globin polyA signal, Phage P1 loxP site, mouse Htr5b genomic DNA
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 2(2):397-406 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Katada Y, Kunimi H, Serizawa N, Lee D, Kobayashi K, Negishi K, Okano H, Tanaka KF, Tsubota K, Kurihara T.
    Starburst amacrine cells amplify optogenetic visual restoration through gap junctions.
    Mol Ther Methods Clin Dev 30 1-13(2023) 37324975