Strain Data Sheet

RBRC05447

Strain Information

Image
BRC No.RBRC05447
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(ACTB-tTS)1Mllo
Former Common nameActin-tTS Tg, B6-ACTB-tTS Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Moises Mallo, Max-Plank Institute of Immunobiology. C57BL/6 and 129 mixed background.
Strain descriptionACTB-tTS transgenic mice. These mice express tetracycline-controlled transcriptional silencer (tTS) gene under the control of the human beta actin promoter. By the combination with homozygous tetO-modified allele, expression from the gene can be blocked in the absence of doxycycline and restore when the antibiotic is administered to the animal.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Reversible gene inactivation in the mouse.
Mallo M, Kanzler B, Ohnemus S
Genomics, 81, 356-360 (2003). 12676559

Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system.
Tanaka K F, Ahmari S E, Leonardo E D, Richardson-Jones J W, Budreck E C, Scheiffele P, Sugio S, Inamura N, Ikenaka K, Hen R
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Oligodendrocytic Na+-K+-Cl- co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain.
Yoshihiko Yamazaki, Yoshifumi Abe, Satoshi Fujii, Kenji F Tanaka
Nat. Commun., 12(1) 5146 (2021). 34446732

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tTS E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNAUNtTS transgene insertion 1, Moises Mallohuman beta-actin promoter, E. coli Tetracycline repressor (tetR), human Zfp KRAB domain
SV40 polyA signalUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman beta actin promoter, tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA), chicken globin genomic DNA, SV40 polyA signal
    Research applicationTet system
    Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEVELOPER (Dr. Moises Mallo, (Max-Plank Institute of Immunobiology) is requested. Genomics, 81, 356-360 (2003). The RECIPIENT must contact the DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEVELOPER and must obtain a prior written consent from the DEVELOPER.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data