Strain Data Sheet

RBRC05448

Strain Information

Image
BRC No.RBRC05448
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;CBB6(129)-Tg(Mlc1-tTS)7Rhn
Former Common nameMlc1-tTS Tg#7, B6-Mlc1-tTS Tg#7
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CBA, C57BL/6 and 129S6 mixed background.
Strain descriptionMlc1-tTS transgenic mice. These transgenic mice express tetracycline-controlled transcriptional silencer (tTS) gene under the control of the mouse Mlc1 promoter. tTS expression is detected in Mlc1 positive astrocytes.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system.
Tanaka K F, Ahmari S E, Leonardo E D, Richardson-Jones J W, Budreck E C, Scheiffele P, Sugio S, Inamura N, Ikenaka K, Hen R
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tTS E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNAUNtTS

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Mlc1 genomic DNA(BAC clone RP23-114I6), tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA), SV40 polyA signal, Phage P1 loxP site
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
    use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka asco-author in the first paper.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data