Strain Data Sheet

RBRC05450

Strain Information

Image
BRC No.RBRC05450
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;CBB6(129)-Tg(Mlc1-tTA)2Rhn
Former Common nameMlc1-tTA#2, B6-Mlc1-tTA Tg#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. C57BL/6 and 129S6 mixed background.
Strain descriptionMlc1-tTA transgenic mice. These transgenic mice express tetracycline-controlled transactivator (tTA) gene under the control of the mouse Mlc1 promoter. tTA expression is detected in Mlc1 positive astrocytes.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system.
Tanaka K F, Ahmari S E, Leonardo E D, Richardson-Jones J W, Budreck E C, Scheiffele P, Sugio S, Inamura N, Ikenaka K, Hen R
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA mouse Mlc1 promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Mlc1 genomic DNA, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 polyA signal
    Research applicationTet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010).
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tsunematsu T, Sakata S, Sanagi T, Tanaka KF, Matsui K.
    Region-Specific and State-Dependent Astrocyte Ca2+ Dynamics during the Sleep-Wake Cycle in Mice.
    J Neurosci 41(25) 5440-5452(2021) 34006590
    Chen J, He W, Hu X, Shen Y, Cao J, Wei Z, Luan Y, He L, Jiang F, Tao Y.
    A role for ErbB signaling in the induction of reactive astrogliosis.
    Cell Discov 3 17044(2017) 29238610