Strain Information | |
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Image | |
BRC No. | RBRC05450 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6;CBB6(129)-Tg(Mlc1-tTA)2Rhn |
Former Common name | Mlc1-tTA#2, B6-Mlc1-tTA Tg#2 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Tanaka, Columbia University in 2007. C57BL/6 and 129S6 mixed background. |
Strain description | Mlc1-tTA transgenic mice. These transgenic mice express tetracycline-controlled transactivator (tTA) gene under the control of the mouse Mlc1 promoter. tTA expression is detected in Mlc1 positive astrocytes. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system. Tanaka K F, Ahmari S E, Leonardo E D, Richardson-Jones J W, Budreck E C, Scheiffele P, Sugio S, Inamura N, Ikenaka K, Hen R Biol. Psychiatry, 67, 770-773 (2010). 20163789 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
tTA | tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain) | UN | tTA | mouse Mlc1 promoter | |||
SV40 polyA signal | UN |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Mlc1 genomic DNA, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 polyA signal |
Research application | Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010). |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Tsunematsu T, Sakata S, Sanagi T, Tanaka KF, Matsui K. Region-Specific and State-Dependent Astrocyte Ca2+ Dynamics during the Sleep-Wake Cycle in Mice. J Neurosci 41(25) 5440-5452(2021) 34006590 |
Chen J, He W, Hu X, Shen Y, Cao J, Wei Z, Luan Y, He L, Jiang F, Tao Y. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3 17044(2017) 29238610 |