Strain Data Sheet

RBRC05451

Strain Information

Image
BRC No.RBRC05451
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nlgn3<tm1(STOP-tetO)Rhn>
Former Common nameNlgn3 STOPtetO KI, B6-Nlgn3/STOPtetO KI
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. C57BL/6 and 129 mixed background.
Strain descriptionNlgn3 knockout mice. Neo-STOP-tetO (loxP-FRT-Neo-STOP-FRT-tetO-loxP) cassette was inserted just upstream of the Nlgn3 gene. Homozygous mutant mice show no obvious abnormality. tet0 mediated allele can be generated by crossing with FLP mice.
Colony maintenanceHomozygote x Hemizygote [or Crossing to C57BL/6JJcl]
References
Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system.
Tanaka K F, Ahmari S E, Leonardo E D, Richardson-Jones J W, Budreck E C, Scheiffele P, Sugio S, Inamura N, Ikenaka K, Hen R
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteXFrt
Frt yeast FRT (flippase recombination target) siteXFrt
Frt yeast FRT (flippase recombination target) siteXFrt
Nlgn3
MGI:2444609		neuroligin 3XNlgn3
  • Asperger syndrome, X-linked, susceptibility to, 1(MedGEN)

  • autism, susceptibility to, X-linked 1(MedGEN)
    • loxP phage P1 loxPXloxP
    loxP phage P1 loxPXloxP
    neo neomycin resistance gene (E. coli)X mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage)
    tetO tetracycline operator (E. coli)XtetO

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal miniature excitatory postsynaptic currents(MP:0004753)

  • abnormal motor coordination/balance(MP:0001516)

  • absent long-term depression(MP:0001899)

  • impaired synaptic plasticity(MP:0001900)

  • increased CNS synapse formation(MP:0004997)
    • more 2 phenotypes
  • increased excitatory postsynaptic current amplitude(MP:0011269)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) sites, mouse PGK promoter, synthetic bacterial EM7 promoter (T7 phage), E. coli neo, yeast His3 3' UTR, SV40 virus SV40 polyA signal, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, mouse Nlgn3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010).
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data