Strain Data Sheet

RBRC05452

Strain Information

Image
BRC No.RBRC05452
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nlgn3<tm1.1(tetO)Rhn>
Former Common nameNlgn3 tetO KI, B6-Nlgn3/tetO KI
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. The mutant mice were backcrossed to 129S6 over 5 times. C57BL/6 and 129 mixed background.
Strain descriptionNlgn3-tetO knock-in mice. tetO was inserted just upstream of the Nlgn3 gene. Expression pattern of the mutant allele is the same as that seen in wild-type mice. By the combination with tTA and tTS, tTA-mediated overexpression and tTS-mediated knockout can be generated respectively in the absence of doxycycline and restore when restore when the antibiotic is administered to the animal.
Colony maintenanceHomozygote x Hemizygote [or Crossing to C57BL/6JJcl]
References
Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system.
Tanaka K F, Ahmari S E, Leonardo E D, Richardson-Jones J W, Budreck E C, Scheiffele P, Sugio S, Inamura N, Ikenaka K, Hen R
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteXFrt
Nlgn3
MGI:2444609		neuroligin 3XNlgn3<tm1.1Rhn> targeted mutation 1.1, Rene Hen
  • Asperger syndrome, X-linked, susceptibility to, 1(MedGEN)

  • autism, susceptibility to, X-linked 1(MedGEN)
    • loxP phage P1 loxPXloxP
    loxP phage P1 loxPXloxP
    tetO tetracycline operator (E. coli)XtetO

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, mouse Nlgn3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010).
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data