Strain Information | |
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Image | |
BRC No. | RBRC05453 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6(Cg)-Tg(tetO-ChR2*C128S/EYFP)1Kftnk |
Former Common name | tetO-ChR2 BAC Tg, B6-tetO-ChR2/YFP Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background. |
Strain description | tetO-ChR2 (C128S) BAC transgenic mice. Linearized modified BAC DNA containing the Actb gene followed by a tet0-ChR2 cassette was injected into C57BL/6 fertilized eggs. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system. Tanaka K F, Matsui K, Sasaki T, Sano H, Sugio S, Fan K, Hen R, Nakai J, Yanagawa Y, Hasuwa H, Okabe M, Deisseroth K, Ikenaka K, Yamanaka A Cell Rep., 2, 397-406 (2012). 22854021 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
ChR2 | channelrhodopsin-2 (Chlamydomonas reinhardtii) | UN | ChR2 | E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron | |||
EYFP | Enhanced Yellow Fluorescent Protein (Aequorea victoria) | UN | EYFP | ||||
Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
loxP | phage P1 loxP | UN | loxP | ||||
loxP | phage P1 loxP | UN | loxP | ||||
loxP | phage P1 loxP | UN | loxP | ||||
loxP | phage P1 loxP | UN | loxP | ||||
SV40 polyA signal | UN |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse beta actin genomic neighborhoods(BAC clone RP23-289L7), Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 virus SV40 polyA signal, yeast FRT (flipase recombination target) site |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 2, 397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper. |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |