Strain Data Sheet

RBRC05453

Strain Information

Image
BRC No.RBRC05453
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Tg(tetO-ChR2*C128S/EYFP)1Kftnk
Former Common nametetO-ChR2 BAC Tg, B6-tetO-ChR2/YFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background.
Strain descriptiontetO-ChR2 (C128S) BAC transgenic mice. Linearized modified BAC DNA containing the Actb gene followed by a tet0-ChR2 cassette was injected into C57BL/6 fertilized eggs.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system.
Tanaka K F, Matsui K, Sasaki T, Sano H, Sugio S, Fan K, Hen R, Nakai J, Yanagawa Y, Hasuwa H, Okabe M, Deisseroth K, Ikenaka K, Yamanaka A
Cell Rep., 2, 397-406 (2012). 22854021

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ChR2 channelrhodopsin-2 (Chlamydomonas reinhardtii)UNChR2 E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron
EYFP Enhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP
Frt yeast FRT (flippase recombination target) siteUNFrt
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse beta actin genomic neighborhoods(BAC clone RP23-289L7), Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 virus SV40 polyA signal, yeast FRT (flipase recombination target) site
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 2, 397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
    use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data