Strain Data Sheet
RBRC05457
Strain Information | |
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| Image | |
| BRC No. | RBRC05457 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.B6CB-Nedd9<tm1Miku> |
| Former Common name | Cas-L KO B6-Nedd9 KO |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Sachiko Seo, Faculty of Medicine, University of Tokyo in 2001. The targeting vector was transferred into TT2 ES cells derived from (C57BL/6 x CBA) F1. The mutant mice were backcrossed to C57BL/6. |
| Strain description | Cas-L (Nedd9, neural precursor cell expressed, developmentally down-regulated gene 9) gene knockout mice. A 1.2 kb region including exon 2 was replaced with EGFP and a floxed neomycin resistant cassette. Homozygous mutant mice show reduced numbers of lymphocytes in secondary lymphoid organs and an almost complete loss of marginal zone B (MZB) cells in the spleen. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Crk-associated substrate lymphocyte type is required for lymphocyte trafficking and marginal zone B cell maintenance. Seo S, Asai T, Saito T, Suzuki T, Morishita Y, Nakamoto T, Ichikawa M, Yamamoto G, Kawazu M, Yamagata T, Sakai R, Mitani K, Ogawa S, Kurokawa M, Chiba S, Hirai H J. Immunol., 175, 3492-3501 (2005). 16148091NEDD9 promotes oncogenic signaling in mammary tumor development. Izumchenko E, Singh M K, Plotnikova O V, Tikhmyanova N, Little J L, Serebriiskii I G, Seo S, Kurokawa M, Egleston B L, Klein-Szanto A, Pugacheva E N, Hardy R R, Wolfson M, Connolly D C, Golemis E A Cancer Res., 69, 7198-7206 (2009). 19738060Crk-associated substrate lymphocyte type regulates myeloid cell motility and suppresses the progression of leukemia induced by p210Bcr/Abl. Seo S, Nakamoto T, Takeshita M, Lu J, Sato T, Suzuki T, Kamikubo Y, Ichikawa M, Noda M, Ogawa S, Honda H, Oda H, Kurokawa M Cancer Sci., 102, 2109-2117 (2011). 21848808 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 13 | EGFP | ||||
| Nedd9 MGI:97302 | neural precursor cell expressed, developmentally down-regulated gene 9 | 13 | Nedd9<tm1Miku> MGI:3700233 | targeted mutation 1, Mineo Kurokawa | |||
| loxP | phage P1 loxP | 13 | loxP | ||||
| loxP | phage P1 loxP | 13 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 13 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | more 4 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, Jellyfish EGFP cDNA, mouse Cas-L (HEF1/NEDD9) genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 175, 3492-3501 (2005). |
| Depositor | Mineo Kurokawa (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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