Strain Data Sheet

RBRC05469

Strain Information

Image
BRC No.RBRC05469
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-H2-Tw3<tm2.1(CAG-EGFP)Maoh>
Former Common nameB6-H2-Tw3/CAG-GFP
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDevelop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain descriptionThe mice having a CAG-EGFP-polyA cassette at the H2-T3 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H
Nucleic Acids Res., 38, e198 (2010). 20880997

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)17EGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Frt yeast FRT (flippase recombination target) site17Frt
H2-T3
MGI:95959		histocompatibility 2, T region locus 317H2-T3<tm2.1(CAG-EGFP)Maoh>
MGI:5056530		targeted mutation 2.1, Masato Ohtsuka
lox2272 phage P1 loxP variant17lox2272
SV40 polyA17

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAGプロモーター, EGFP, SV40 polyA, FRT, lox2272
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasato　Ohtsuka (Tokai University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data